I agree with MA and Kat.You need a more light if you like to change aperture for bigger DOF. I do some test with my 50 mm F:1.7 and ISO 1600 which I never use.For even more light I set camera on +2EV and WB on tungsten.Aperture on 2.8 to 4 with manual focus.Image looks perfectly exposed and DOF is just enough wide for sharp eyes.My shutter speed is 1/15 sec.I not use tripod.I take image of one word on door from angle of 45 degrees.
It is possible do it this way but for better quality (noise) and for better DOF use some lights.